Tag: method

  • New method for early detection of SARS-CoV-2 infection

    New method for early detection of SARS-CoV-2 infection

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    New Delhi: A new sandwich-based lateral flow immunoassay (LFIA) for the detection of Receptor Binding Domain (RBD) of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) could provide an efficient substitute for RT-PCR tests.

    According to Union Ministry of Science and Technology, it can detect the RBD antigen of the SARS-CoV-2 at an early stage of infection with a visual line of detection (LOD).

    Popularly used gold standard techniques such as RT-PCR and ELISA are usually time-consuming, require skilled labor, specific equipment and are not feasible for on-site detection.

    MS Education Academy

    In order to overcome this challenge, a team of researchers from DBT-National Institute of Animal Biotechnology (NIAB) and Gandhi Medical College developed a rapid and robust platform for early and on-field detection of SARS-CoV-2 virus.

    A smartphone app (Colour grab) has been used for the qualitative analysis of the test strip. The developed LFIA working on the principle of antigen-antibody interaction holds the potential to be used for detection of SARS-CoV-2 without any requirement of skilled personnel and subsequently reduce the spread of the virus.

    Officials said that with support from Science and Engineering Research Board (SERB), an institution of the Department of Science and Technology (DST) the scientists came together to clone the gene responsible for RBD protein expression, and purify it to generate the antibody (Ab). The generated antibodies were then conjugated with monodisperse gold nanoparticles (AuNPs) to be used as a capture probe for colorimetric detection.

    The fabricated LFIA works in a sandwich format, where the RBD target analyte in the sample interacts with the gold nanoparticle conjugated RBD antibody to form a complex (AuNPs-Ab), which further moves along the nitrocellulose membrane and reacts with the RBD antibody coated as test line on the membrane to form a red colour band.

    The RBD Ab and IgG secondary Ab were coated on the nitrocellulose membrane as test and control, respectively to test the presence of antigen (Ag) in the sample. Further, the excess antibody bonded with the control line coated with a secondary IgG antibody validating the functionality of the test strip. An absorbent pad was placed at the end of the strip to prevent backflow across the strip.

    The presence of band colour at test and control line indicates a positive result whereas a single line in control indicates a negative result. Depending on the target analyte, various parameters such as blocking buffer, antibody conjugation concentration, and antibody coating concentration on the membrane have been optimised to obtain band color with maximum binding efficiency.

    The band intensity of the test line was analysed using a simple smartphone-based application for image acquisition and analysis of the test line color, which can split any color data into its three primary color components — red, green, and blue (RGB data). The specific colour component shows the trend of increasing or decreasing intensity of color. This study was published in the Journal of Medical Virology recently.

    Officials said that the developed LFIA strips can be useful as a portable, point of care device (PoC) for on-site detection of SARS-CoV-2 particularly atA home or even in rural areas. Moreover, the cost of LFIA strips is much less as compared to standard RT-PCR test making it a more economical option for people who cannot afford the RT-PCR test.

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    #method #early #detection #SARSCoV2 #infection

    ( With inputs from www.siasat.com )

  • Jamia Nagar violence: Chakka jam not violent protest method, Sharjeel Imam tells HC

    Jamia Nagar violence: Chakka jam not violent protest method, Sharjeel Imam tells HC

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    New Delhi: Defending his discharge in a 2019 Jamia Nagar violence case, JNU student Sharjeel Imam Thursday told the Delhi High Court he only campaigned in favour of peaceful protest and ‘chakka jam’ cannot be termed a “violent method of protest”.

    Imam’s stand came in his written submissions filed in response to the Delhi Police’s plea challenging the trial court order of February 4 discharging him and several others, including student activists Asif Iqbal Tanha and Safoora Zargar, in the matter.

    The case concerns the violence that erupted after a clash between police and people protesting against the Citizenship Amendment Act (CAA) in the Jamia Nagar area here in December 2019.

    Justice Swarana Kanta Sharma listed the case for hearing on March 23 after the investigating agency sought time on account of the ill health of one of its lawyers.

    The court asked the respondents to file their written submissions in the meantime.

    It also directed that the electronic evidence, including videos, be placed on record.

    The trial court had in its February 4 order discharged 11 people in the case while holding that they were made “scapegoats” by police and that dissent has to be encouraged not stifled.

    The police, in its revision petition, has said the trial court’s order is in the teeth of well settled principles of law, suffers from grave infirmities, and is perverse.

    The police plea said the trial court got swayed by “emotional and sentimental feelings” and cast aspersions on the prosecuting agency. It passed “gravely prejudicial” and “adverse” remarks against the prosecuting agency and the investigation, the revision petition says.

    In his written submissions, Imam said shouting slogans in favour of a particular means of peaceful protest in no way portrays his participation in the violence that ensued later.

    Imam was accused of instigating the riots by delivering a provocative speech at the Jamia Milia University on December 13, 2019. He continues to remain in jail as he is an accused in the larger conspiracy case of the 2020 northeast Delhi riots.

    He stated the protesters had assembled in exercise of their fundamental right to assemble peacefully as guaranteed under the Constitution, and in absence of any prohibitory orders, no culpability could be attributed to him.

    “In his speech at AMU on 16.12.2019, the Answering Respondent merely stated that he campaigned in favor of chakka jam as a means of protest which by no stretch of the imagination could be called a violent method of protest,” he submitted.

    There is no admissible evidence against Imam to show that he shared the common object of the unlawful assembly and that “he was a victim of the violence and had no active role to play in its culmination”, he said in the written submissions.

    “The Answering Respondent is not to be seen in any of the videos adduced by the Prosecution nor is there any statement recorded by the investigating agency in which he is even named much less having attributed any role to him in the commission of the alleged violence,” it stated.

    The fact that violence occurred during the peaceful protest at Jamia which resulted in Sharjeel Imam breaking his glasses is not indicative of the fact that the he participated in the said violence, he has submitted.

    Imam also asserted that his CDR clearly established that he had already left the place of occurrence before the assembly turned unlawful and the allegedly provocative speech was delivered much after the alleged rioting and is subject matter of another case.

    “(Imam) only campaigned in favour of a means of peaceful protest, not violence. The act of shouting slogans in favour of a particular means of peaceful protest in no way portrays the participation of the Answering Respondent in the violence that ensued during the protest,” he said.

    The alleged disclosure statement by Imam, the document said, was not recorded by the investigating agency in the present case and even otherwise it has got no evidentiary value.

    On February 13, the high court had issued notice to Imam and others on the police plea, and clarified that the observations of the lower court would not affect further investigation in the matter or trial of any accused.

    Additional Solicitor General Sanjay Jain, representing Delhi Police, has urged the high court to expunge the remarks made by the trial court, arguing that the case concerned an unlawful assembly turning violent and not the “right to dissent”.

    The police said at the stage of consideration of an application for discharge, the trial court has to proceed with an assumption that the materials brought on record by the prosecution are true and not go deep into the matter as if to decide the issue of conviction.

    Noting that the accused were merely present at the protest site and there was no incriminating evidence against them, the trial court had said dissent is an extension of the fundamental right to freedom of speech and expression, subject to reasonable restrictions.

    While discharging the 11 accused, the trial court had ordered framing of charges against one of them- Mohammad Ilyas.

    The Jamia Nagar police station had filed charge sheet under several provisions of the Indian Penal Code against Imam, Tanha, Zargar, Mohammad Qasim, Mahmood Anwar, Shahzar Raza Khan, Mohammad Abuzar, Mohammad Shoaib, Umair Ahmad, Bilal Nadeem, Chanda Yadav and Mohammad Ilyas.

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    #Jamia #Nagar #violence #Chakka #jam #violent #protest #method #Sharjeel #Imam #tells

    ( With inputs from www.siasat.com )